PhanerochaeteV1, Main, Exploration, bibRecord, 000592

[Molecular cloning and expression of alcohol dehydrogenase gene of Phanerochaete chrysosporium].

Identifieur interne : 000592 ( Main/Exploration ); précédent : 000591; suivant : 000593

[Molecular cloning and expression of alcohol dehydrogenase gene of Phanerochaete chrysosporium].

Auteurs : Chuan He [République populaire de Chine] ; Jin-Ming Wu ; Xi-Gen Zhang ; Bo Wu ; Yi-Zheng Zhang

Source :

Yi chuan = Hereditas [ 0253-9772 ] ; 2009.

RBID : pubmed:19586851

Descripteurs français

English descriptors

KwdEn :
- Alcohol Dehydrogenase (genetics), Alcohol Dehydrogenase (metabolism), Amino Acid Sequence (MeSH), Base Sequence (MeSH), Cloning, Molecular (MeSH), DNA, Complementary (MeSH), DNA, Fungal (analysis), Gene Expression (MeSH), Gene Expression Regulation, Fungal (MeSH), Molecular Sequence Data (MeSH), Phanerochaete (genetics), Phanerochaete (metabolism), Recombinant Proteins (genetics), Recombinant Proteins (metabolism), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Sequence Alignment (MeSH), Sequence Analysis, DNA (MeSH), Sequence Homology, Amino Acid (MeSH).
MESH :
- chemical , analysis : DNA, Fungal.
- chemical , genetics : Alcohol Dehydrogenase, Recombinant Proteins.
- chemical , metabolism : Alcohol Dehydrogenase, Recombinant Proteins.
- genetics : Phanerochaete.
- metabolism : Phanerochaete.
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Complementary, Gene Expression, Gene Expression Regulation, Fungal, Molecular Sequence Data, Reverse Transcriptase Polymerase Chain Reaction, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid.

Abstract

When Phanerochaete chrysosporium is grown under oxygen-limited condition, ethanol is one of the important metabolites. In order to understand the metabolic mechanism of P. chrysosporium grown under oxygen-limited condition, a cDNA sequence (1 071 bp) designated "PCAdh1" encoding an alcohol dehydrogenase (ADH) was cloned from the filamentous white-rot fungus P. chrysosporium. PCAdh1 gene encodes a protein of 356 amino acid residues. Although the catalytic domain and coenzyme-binding domain were highly conserved, the protein sequence of PCAdh1 showed a low level of similarity to other known ADH. The recombinant PCAdh1 protein was expressed in Escherichia coli and its enzyme activity was detected. The protein was purified and used to prepare antibody. Semi-quantitative RT-PCR and Western blot demonstrated that the expression level of PCAdh1 in P. chrysosporium remained stable despite the lowered oxygen content, indicating that the gene expression is constitutive. But with the reduction of oxygen content, the overall activity of ADH from the crude mycelia proteins was increased during the growing periods, implying that the expression of other Adh genes in P. chrysosporium is inductive.

DOI: 10.3724/sp.j.1005.2009.00546
PubMed: 19586851

Affiliations:

République populaire de Chine

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Le document en format XML

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<author><name sortKey="He, Chuan" sort="He, Chuan" uniqKey="He C" first="Chuan" last="He">Chuan He</name>
<affiliation wicri:level="1"><nlm:affiliation>Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, Chengdu 610064, China. chuanhe_1985@yahoo.com.cn</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, Chengdu 610064</wicri:regionArea>
<wicri:noRegion>Chengdu 610064</wicri:noRegion>
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<author><name sortKey="Wu, Jin Ming" sort="Wu, Jin Ming" uniqKey="Wu J" first="Jin-Ming" last="Wu">Jin-Ming Wu</name>
</author>
<author><name sortKey="Zhang, Xi Gen" sort="Zhang, Xi Gen" uniqKey="Zhang X" first="Xi-Gen" last="Zhang">Xi-Gen Zhang</name>
</author>
<author><name sortKey="Wu, Bo" sort="Wu, Bo" uniqKey="Wu B" first="Bo" last="Wu">Bo Wu</name>
</author>
<author><name sortKey="Zhang, Yi Zheng" sort="Zhang, Yi Zheng" uniqKey="Zhang Y" first="Yi-Zheng" last="Zhang">Yi-Zheng Zhang</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">[Molecular cloning and expression of alcohol dehydrogenase gene of Phanerochaete chrysosporium].</title>
<author><name sortKey="He, Chuan" sort="He, Chuan" uniqKey="He C" first="Chuan" last="He">Chuan He</name>
<affiliation wicri:level="1"><nlm:affiliation>Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, Chengdu 610064, China. chuanhe_1985@yahoo.com.cn</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, Chengdu 610064</wicri:regionArea>
<wicri:noRegion>Chengdu 610064</wicri:noRegion>
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<author><name sortKey="Wu, Jin Ming" sort="Wu, Jin Ming" uniqKey="Wu J" first="Jin-Ming" last="Wu">Jin-Ming Wu</name>
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<author><name sortKey="Zhang, Xi Gen" sort="Zhang, Xi Gen" uniqKey="Zhang X" first="Xi-Gen" last="Zhang">Xi-Gen Zhang</name>
</author>
<author><name sortKey="Wu, Bo" sort="Wu, Bo" uniqKey="Wu B" first="Bo" last="Wu">Bo Wu</name>
</author>
<author><name sortKey="Zhang, Yi Zheng" sort="Zhang, Yi Zheng" uniqKey="Zhang Y" first="Yi-Zheng" last="Zhang">Yi-Zheng Zhang</name>
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<series><title level="j">Yi chuan = Hereditas</title>
<idno type="ISSN">0253-9772</idno>
<imprint><date when="2009" type="published">2009</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alcohol Dehydrogenase (genetics)</term>
<term>Alcohol Dehydrogenase (metabolism)</term>
<term>Amino Acid Sequence (MeSH)</term>
<term>Base Sequence (MeSH)</term>
<term>Cloning, Molecular (MeSH)</term>
<term>DNA, Complementary (MeSH)</term>
<term>DNA, Fungal (analysis)</term>
<term>Gene Expression (MeSH)</term>
<term>Gene Expression Regulation, Fungal (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Phanerochaete (genetics)</term>
<term>Phanerochaete (metabolism)</term>
<term>Recombinant Proteins (genetics)</term>
<term>Recombinant Proteins (metabolism)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term>
<term>Sequence Alignment (MeSH)</term>
<term>Sequence Analysis, DNA (MeSH)</term>
<term>Sequence Homology, Amino Acid (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (MeSH)</term>
<term>ADN fongique (analyse)</term>
<term>Alcohol dehydrogenase (génétique)</term>
<term>Alcohol dehydrogenase (métabolisme)</term>
<term>Alignement de séquences (MeSH)</term>
<term>Analyse de séquence d'ADN (MeSH)</term>
<term>Clonage moléculaire (MeSH)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Expression des gènes (MeSH)</term>
<term>Phanerochaete (génétique)</term>
<term>Phanerochaete (métabolisme)</term>
<term>Protéines recombinantes (génétique)</term>
<term>Protéines recombinantes (métabolisme)</term>
<term>RT-PCR (MeSH)</term>
<term>Régulation de l'expression des gènes fongiques (MeSH)</term>
<term>Similitude de séquences d'acides aminés (MeSH)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>Séquence nucléotidique (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Fungal</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Alcohol Dehydrogenase</term>
<term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Alcohol Dehydrogenase</term>
<term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN fongique</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phanerochaete</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Alcohol dehydrogenase</term>
<term>Phanerochaete</term>
<term>Protéines recombinantes</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Phanerochaete</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Alcohol dehydrogenase</term>
<term>Phanerochaete</term>
<term>Protéines recombinantes</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Base Sequence</term>
<term>Cloning, Molecular</term>
<term>DNA, Complementary</term>
<term>Gene Expression</term>
<term>Gene Expression Regulation, Fungal</term>
<term>Molecular Sequence Data</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>Sequence Alignment</term>
<term>Sequence Analysis, DNA</term>
<term>Sequence Homology, Amino Acid</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>ADN complémentaire</term>
<term>Alignement de séquences</term>
<term>Analyse de séquence d'ADN</term>
<term>Clonage moléculaire</term>
<term>Données de séquences moléculaires</term>
<term>Expression des gènes</term>
<term>RT-PCR</term>
<term>Régulation de l'expression des gènes fongiques</term>
<term>Similitude de séquences d'acides aminés</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
</keywords>
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<front><div type="abstract" xml:lang="en">When Phanerochaete chrysosporium is grown under oxygen-limited condition, ethanol is one of the important metabolites. In order to understand the metabolic mechanism of P. chrysosporium grown under oxygen-limited condition, a cDNA sequence (1 071 bp) designated "PCAdh1" encoding an alcohol dehydrogenase (ADH) was cloned from the filamentous white-rot fungus P. chrysosporium. PCAdh1 gene encodes a protein of 356 amino acid residues. Although the catalytic domain and coenzyme-binding domain were highly conserved, the protein sequence of PCAdh1 showed a low level of similarity to other known ADH. The recombinant PCAdh1 protein was expressed in Escherichia coli and its enzyme activity was detected. The protein was purified and used to prepare antibody. Semi-quantitative RT-PCR and Western blot demonstrated that the expression level of PCAdh1 in P. chrysosporium remained stable despite the lowered oxygen content, indicating that the gene expression is constitutive. But with the reduction of oxygen content, the overall activity of ADH from the crude mycelia proteins was increased during the growing periods, implying that the expression of other Adh genes in P. chrysosporium is inductive.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19586851</PMID>
<DateCompleted><Year>2009</Year>
<Month>09</Month>
<Day>22</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>09</Month>
<Day>23</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0253-9772</ISSN>
<JournalIssue CitedMedium="Print"><Volume>31</Volume>
<Issue>5</Issue>
<PubDate><Year>2009</Year>
<Month>May</Month>
</PubDate>
</JournalIssue>
<Title>Yi chuan = Hereditas</Title>
<ISOAbbreviation>Yi Chuan</ISOAbbreviation>
</Journal>
<ArticleTitle>[Molecular cloning and expression of alcohol dehydrogenase gene of Phanerochaete chrysosporium].</ArticleTitle>
<Pagination><MedlinePgn>546-51</MedlinePgn>
</Pagination>
<Abstract><AbstractText>When Phanerochaete chrysosporium is grown under oxygen-limited condition, ethanol is one of the important metabolites. In order to understand the metabolic mechanism of P. chrysosporium grown under oxygen-limited condition, a cDNA sequence (1 071 bp) designated "PCAdh1" encoding an alcohol dehydrogenase (ADH) was cloned from the filamentous white-rot fungus P. chrysosporium. PCAdh1 gene encodes a protein of 356 amino acid residues. Although the catalytic domain and coenzyme-binding domain were highly conserved, the protein sequence of PCAdh1 showed a low level of similarity to other known ADH. The recombinant PCAdh1 protein was expressed in Escherichia coli and its enzyme activity was detected. The protein was purified and used to prepare antibody. Semi-quantitative RT-PCR and Western blot demonstrated that the expression level of PCAdh1 in P. chrysosporium remained stable despite the lowered oxygen content, indicating that the gene expression is constitutive. But with the reduction of oxygen content, the overall activity of ADH from the crude mycelia proteins was increased during the growing periods, implying that the expression of other Adh genes in P. chrysosporium is inductive.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>He</LastName>
<ForeName>Chuan</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, Chengdu 610064, China. chuanhe_1985@yahoo.com.cn</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wu</LastName>
<ForeName>Jin-Ming</ForeName>
<Initials>JM</Initials>
</Author>
<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Xi-Gen</ForeName>
<Initials>XG</Initials>
</Author>
<Author ValidYN="Y"><LastName>Wu</LastName>
<ForeName>Bo</ForeName>
<Initials>B</Initials>
</Author>
<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Yi-Zheng</ForeName>
<Initials>YZ</Initials>
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<Language>chi</Language>
<PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType>
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<MedlineJournalInfo><Country>China</Country>
<MedlineTA>Yi Chuan</MedlineTA>
<NlmUniqueID>9436478</NlmUniqueID>
<ISSNLinking>0253-9772</ISSNLinking>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D004271">DNA, Fungal</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
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<Chemical><RegistryNumber>EC 1.1.1.1</RegistryNumber>
<NameOfSubstance UI="D000426">Alcohol Dehydrogenase</NameOfSubstance>
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<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000426" MajorTopicYN="N">Alcohol Dehydrogenase</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004271" MajorTopicYN="N">DNA, Fungal</DescriptorName>
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</MeshHeading>
<MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015966" MajorTopicYN="N">Gene Expression Regulation, Fungal</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017386" MajorTopicYN="N">Sequence Homology, Amino Acid</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
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<Month>7</Month>
<Day>10</Day>
<Hour>9</Hour>
<Minute>0</Minute>
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<PubMedPubDate PubStatus="pubmed"><Year>2009</Year>
<Month>7</Month>
<Day>10</Day>
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<PubMedPubDate PubStatus="medline"><Year>2009</Year>
<Month>9</Month>
<Day>23</Day>
<Hour>6</Hour>
<Minute>0</Minute>
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<PublicationStatus>ppublish</PublicationStatus>
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<ArticleId IdType="pii">0253-9772(2009)05-546-6</ArticleId>
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<tree><noCountry><name sortKey="Wu, Bo" sort="Wu, Bo" uniqKey="Wu B" first="Bo" last="Wu">Bo Wu</name>
<name sortKey="Wu, Jin Ming" sort="Wu, Jin Ming" uniqKey="Wu J" first="Jin-Ming" last="Wu">Jin-Ming Wu</name>
<name sortKey="Zhang, Xi Gen" sort="Zhang, Xi Gen" uniqKey="Zhang X" first="Xi-Gen" last="Zhang">Xi-Gen Zhang</name>
<name sortKey="Zhang, Yi Zheng" sort="Zhang, Yi Zheng" uniqKey="Zhang Y" first="Yi-Zheng" last="Zhang">Yi-Zheng Zhang</name>
</noCountry>
<country name="République populaire de Chine"><noRegion><name sortKey="He, Chuan" sort="He, Chuan" uniqKey="He C" first="Chuan" last="He">Chuan He</name>
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[Molecular cloning and expression of alcohol dehydrogenase gene of Phanerochaete chrysosporium].

[Molecular cloning and expression of alcohol dehydrogenase gene of Phanerochaete chrysosporium].

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki